Shown below is the output of the command:
enclone BCR=123085 GEX=123217 LVARSP=gex,IGHV2-5_g_μ CDR3=CALMGTYCSGDNCYSWFDPW
[1] GROUP = 1 CLONOTYPES = 6 CELLS
[1.1] CLONOTYPE = 6 CELLS
┌──────────────────────────┬───────────────────────────────────────┬─────────────────────────────┐
│ │ CHAIN 1 │ CHAIN 2 │
│ │ 98|IGHV2-5 ◆ 13|IGHD2-15 ◆ 57|IGHJ5 │ 349|IGLV3-1 ◆ 311|IGLJ2 │
│ ├───────────────────────────────────────┼─────────────────────────────┤
│ │ 11111111111111111111 │ 11111111111 │
│ │ 8 11111222222222233333 │ 6 00000111111 │
│ │ 5 56789012345678901234 │ 2 56789012345 │
│ │ ════════CDR3════════ │ ════CDR3═══ │
│reference │ S ◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦W │ V CQAWD◦◦◦◦◦◦ │
│donor ref │ S ◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦W │ V CQAWD◦◦◦◦◦◦ │
├──────────────────────────┼───────────────────────────────────────┼─────────────────────────────┤
│# n gex IGHV2-5_g_μ │ x ..x.......xx........ u const │ x ........... u const│
│1 3 9011 1849 │ S CALMGTYCSGDNCYSWFDPW 592 IGHM │ V CQAWDSSVVVF 2995 IGLC2│
│2 1 29846 6515 │ S CALMGTYCSGDNCYSWFDPW 6218 IGHG1 │ V CQAWDSSVVVF 15182 IGLC2│
│3 1 14995 3326 │ T CALMGTYCSGDNCYSWFDPW 4033 IGHG1 │ V CQAWDSSVVVF 6777 IGLC2│
│4 1 3250 2 │ S CAHMGTYCSGGSCYSWFDPW 18 IGHG1 │ V CQAWDSSVVVF 592 IGLC2│
└──────────────────────────┴───────────────────────────────────────┴─────────────────────────────┘
This shows an invocation of enclone that takes VDJ, and gene expression data as input, and
exhibits all clonotypes for which some chain has the given CDR3 sequence. As well the command
requests UMI (molecule) counts for one hand-selected gene. You can use any gene(s) you like and
any antibodies for which you have feature barcodes.