enclone banner

Shown below is the output of the command:

enclone BCR=123085 GEX=123217 LVARSP=gex,IGHV2-5_g_μ CDR3=CALMGTYCSGDNCYSWFDPW

[1] GROUP = 1 CLONOTYPES = 6 CELLS

[1.1] CLONOTYPE = 6 CELLS
┌──────────────────────────┬───────────────────────────────────────┬─────────────────────────────┐
│                          │  CHAIN 1                              │  CHAIN 2                    │
│                          │  98|IGHV2-5 ◆ 13|IGHD2-15 ◆ 57|IGHJ5  │  349|IGLV3-1 ◆ 311|IGLJ2    │
│                          ├───────────────────────────────────────┼─────────────────────────────┤
│                          │    11111111111111111111               │    11111111111              │
│                          │  8 11111222222222233333               │  6 00000111111              │
│                          │  5 56789012345678901234               │  2 56789012345              │
│                          │    ════════CDR3════════               │    ════CDR3═══              │
│reference                 │  S ◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦WV CQAWD◦◦◦◦◦◦              │
│donor ref                 │  S ◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦◦WV CQAWD◦◦◦◦◦◦              │
├──────────────────────────┼───────────────────────────────────────┼─────────────────────────────┤
│#  n    gex  IGHV2-5_g_μ    x ..x.......xx........     u  const    x ...........      u  const│
│1  3   9011         1849  │  S CALMGTYCSGDNCYSWFDPW   592  IGHM   │  V CQAWDSSVVVF   2995  IGLC2│
│2  1  29846         6515  │  S CALMGTYCSGDNCYSWFDPW  6218  IGHG1  │  V CQAWDSSVVVF  15182  IGLC2│
│3  1  14995         3326  │  T CALMGTYCSGDNCYSWFDPW  4033  IGHG1  │  V CQAWDSSVVVF   6777  IGLC2│
│4  1   3250            2  │  S CAHMGTYCSGGSCYSWFDPW    18  IGHG1  │  V CQAWDSSVVVF    592  IGLC2│
└──────────────────────────┴───────────────────────────────────────┴─────────────────────────────┘

This shows an invocation of enclone that takes VDJ, and gene expression data as input, and
exhibits all clonotypes for which some chain has the given CDR3 sequence.  As well the command
requests UMI (molecule) counts for one hand-selected gene.  You can use any gene(s) you like and
any antibodies for which you have feature barcodes.